In vitro studies on antidiabetic, antioxidant and phytochemical activities of endophytic actinomycete from Azadirachta indica A. Juss
نویسندگان
چکیده
Inhibitory effects of root endophytic actinomycete A-12 from Azadirachta indica A. Juss (Indian lilac, neem) against α-amylase and α-glucosidase were studied. The antioxidant capability was assessed through 2,2-diphenyl-picrylhydrazyl (DPPH), hydroxy radical scavenging and reducing power assays. In vitro antidiabetic activity of ethyl acetate extract revealed good inhibition exhibiting IC50 values of 411.70 and 208.92 μg/ml against α-amylase and α-glucosidase respectively. The reducing power of the extract was found to be concentration dependent. The extract revealed measurable total phenolics i.e., 80.22±9.67 (catechol) and 12.48±1.96 mg/g (gallic acid) equivalents. The extract exhibited remarkable scavenging activity on DPPH (IC50=447.95±0.35μg/ml) and hydroxyl radical (IC50=1961.02±0.69μg/ml) and also displayed high and dose-dependent antioxidant capacity. The results are therefore indicative of the possible potentials of ethyl acetate extracts to manage hyperglycemia.
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